Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using solid phase extraction (SPE) technique for the determination of azilsartan and amlodipine in human plasma; chromatographic separation of the analyte was achieved at 7.61 min and 18.49 min for amlodipine and azilsartan respectively. HPLC column C 18, 150 X 4.6, 5µ, flow rate was 1.2 ml/ min, column oven temperature 25°C, injection volume 50 µl and mobile phase buffer was 50 mM potassium dihydrogen ortho phosphate buffer pH 7.00 and the detection was carried out at 240 nm. Calibration curve was linear (r2>0.9998) in the range of 10-60 ng/ml and 25-150 ng/ml for amlodipine and azilsartan respectively. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC method was successfully validated and may be applied to conduct bioavailability and bioequivalence studies.
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